Nanoscale label-free bioprobes to detect intracellular proteins in single living cells

Citation:

Hong W, Liang F, Schaak D, Loncar M, Quan Q. Nanoscale label-free bioprobes to detect intracellular proteins in single living cells [Internet]. Scientific Reports 2014;4:6179.
srep06179.pdf2.28 MB

Abstract:

Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dynamics through a nanoscale surface plasmon-polariton fiber-tip-probe (FTP).

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Last updated on 10/30/2015