Ethan Schonbrun

Optofluidic Cytometry 


Rowland Junior Fellow
Rowland Institute at Harvard
Harvard University
100 Edwin H. Land Blvd.
Cambridge, MA 02142
Tel: 617-497-4xxx
Fax: 617-497-4627
Email: schonbrun@rowland.harvard.edu
Website: Optofluidic Cytometry Lab

Our group develops optical technologies for capturing 2D, 3D and hyperspectral images of cells in fast moving fluids.  These systems exploit spectral, angular and polarization degrees of freedom in the optical signal, as well as recent advances in microoptics, high speed light sources and cameras. In addition to spatial and spectral information, we are also interested in performing quantitative measurements of other physical characteristics of cells, such as volume, protein mass, and oxygen tension.  Resolving the distribution of various measurable parameters enables better understanding of subtle differences in the response of each cell to its environment. We are currently using these measurements to differentiate pathological blood cells and to study the life cycle and kinetics of immune cells.

Selected Publications

  • E. Schonbrun, R. Malka, G. Di Caprio, D. Schaak, and J. M. Higgins, “Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume,” J. Cytometry, 85, 332-338 (2014).

  • E. Schonbrun, G. Moller, and G. Di Caprio, “Polarization encoded color camera,” Opt. Lett., 39, 1433-1436 (2014).

  • G. Di Caprio, D. Schaak, and E. Schonbrun, “Hyperspectral fluorescence microfluidic microscopy,” Biomed. Opt. Exp. 4, 1486-1493 (2013).

  • E. Schonbrun, G. Di Caprio, and D. Schaak, “Dye exclusion microfluidic microscopy,” Opt. Exp. 21, 8793-8798 (2013).

  • S. S. Gorthi, D. Schaak and E. Schonbrun, "Fluorescence imaging of flowing cells using a temporally coded excitation,” Opt. Exp. 21, 5164-5170 (2013).

  • S. S. Gorthi and E. Schonbrun, "Phase imaging flow cytometry," Opt. Lett. 37, 707-709 (2012).

  • E. Schonbrun, S. S. Gorthi, and D. Schaak, "Microfabricated multiple field of view imaging flow cytometry," Lab on a Chip. 12, 268-273 (2012).