Alvaro Sanchez

Microbial Biophysics and Systems Biology Lab

Rowland Junior Fellow
Rowland Institute at Harvard
Harvard University
100 Edwin H. Land Blvd.
Cambridge, MA 02142
Tel: 617-497-4780
Fax: 617-497-4627
Email: sanchez@rowland.harvard.edu
Website: Microbial Biophysics and Systems Biology Lab

Microbes are social organisms that are most commonly found forming complex ecological communities. Every species in a community is typically represented by large numbers of cells which may also differ in their individual behaviors. A fascinating fact is that the individual behavior of a microbial cell often depends on what the other cells in their vicinity are doing; much like animals do, microbes implement social strategies. Not having a central nervous system to help them make decisions, bacteria adopt these social strategies as alternative phenotypes, encoded in their DNA sequences and governed by biochemical and gene regulatory circuits. Evolutionary changes in these "decision-making circuits" will therefore affect ecological interactions between species and are an essential component of microbial communities. Our lab is interested in shedding some light into this rather complex system, and understanding the relationship between the evolution of microbial behavior and microbial ecology. For instance, we ask questions such as: How is the social behavior of microbes encoded in their DNA? How does the behavior of individuals from one species affect the behavior of other species in the community? Can we describe these interactions quantitatively? How does the structure of microbial communities affect the evolution of social traits? Can we quantitatively map complex microbial interaction networks and predict their community dynamics? In order to tackle these questions, we use a combination of biophysical tools (in order to grow large numbers of cultures in parallel and help us determine the frequencies of different species in these communities), mathematical and computational modeling (to help us generate quantitative hypotheses and understand our data) and systems and synthetic biology (in order to engineer cells with pre-determined social behaviors and to assemble communities in vitro).

Selected Publications

  • Feedback between population and evolutionary dynamics determines the fate of social microbial populations (2013) Alvaro Sanchez and Jeff Gore, PLOS Biology 11(4): e1001547
  • Regulation of noise in gene expression (2013) Alvaro Sanchez, Sandeep Choubey and Jane Kondev, Annual Reviews in Biophysics 42: 469–491
  • Operator sequence alters gene expression independently of transcription factor occupancy in bacteria (2012) Hernan G. Garcia*, Alvaro Sanchez*, James Q. Boedicker*, Melisa L. Osborne, Jeff Gelles, Jane Kondev and Rob Phillips;Cell Reports 2(1): 150-161  (*shared first authorship)
  • Mechanism of transcriptional repression at a bacterial promoter by analysis of single molecules (2011) Alvaro Sanchez, Melisa Osborne, Larry Friedman, Jane Kondev and Jeff Gelles,  EMBO Journal 30:3940-3946
  • Effect of promoter architecture on the cell-to-cell variability in gene expression (2011) Alvaro Sanchez, Hernan G. Garcia, Daniel Jones, Rob Phillips and Jane Kondev, PLOS Computational Biology 7(3):e1001100
  • Transcriptional control of noise in gene expression (2008) Alvaro Sanchez and Jane Kondev,  PNAS 105(13):5081-6.